Simultaneous determination of polyphenol content Vitis amurensis Rupr. by tandem mass spectrometry

Vitis amurensis Ruprecht contains a large number of polyphenolic compounds which are biologically active components. For the most efficient and safe extraction supercritical carbon dioxide was used. In this work, for first time, comparative metabolomic study substances wild grapes collected from five different places Primorsky Khabarovsk territories is carried out. To identify target analytes in ethanol extracts grape berries, high performance liquid chromatography (HPLC) used combination with an amaZon SL ion trap (manufactured by BRUKER DALTONIKS, Germany) equipped ESI electrospray ionization source negative positive modes. The mass spectrometer scan range m / z 100 - 1.700 MS MS. Used fragmentation 4 th order. Primary spectrometric results showed presence 94 corresponding to species V. , moreover, salvianolic acids F, D G, oleanoic, ursolic, myristoleic acids, berbericinin, mearnsetin, esculin, nevadensin, stigmasterol, fucosterol, phlorizin, L-tryptophan identified time .